特邀学术报告 - Targeting RNA sequences and structures with a PNA-based  programmable platform

审核发布:群体微生物研究中心 来源单位及审核人: 发布时间:2019-11-15浏览次数:374

特邀学术报告

 

    间:20191118日下午330

    点:资环楼105 室(芭田报告厅)

人:陈刚 助理教授

报告题目:Targeting RNA sequences and structures with a

         PNA-based programmable platform

  人:刘定祥 教授

  

个人简介:

陈刚博士,2001年本科毕业于中国科技大学,后赴美国罗切斯特大学化学系攻读博士学位。曾就职于加州大学伯克利分校化学系和Scripps研究所。2010年起至今就职于新加坡南洋理工大学数理科学学院化学与生物化学中心。

  

 

Abstract

RNAs perform a diverse array of catalytic and regulatory functions in viruses and cells and are becoming increasingly important disease biomarkers and drug targets. RNA structures are mainly stabilized by base paired double-stranded (ds) stem regions. Together with single-stranded (ss) loop regions, RNAs can fold into complex secondary and tertiary structures, facilitating the molecular recognition of RNA structures by small molecules and peptides/proteins. Currently, programmable RNA structure-specific and tight-binding ligands are relatively unexplored. Peptide nucleic acid (PNA) is characterized by a neutral, peptide-like backbone, with superior chemical stability and strong binding to complementary RNA/DNA sequences. Importantly, PNAs conjugated with cell-penetrating moieties show promising bioactivities in animal models. In this presentation, I will present our results on the synthesis and biophysical characterization of the novel chemically-modified dsRNA-binding PNAs (dbPNAs), which show selective recognition of dsRNAs over ssRNAs and dsDNAs in a sequence-specific manner. dbPNAs and traditional antisense PNAs (asPNAs) can be combined to  serve as useful dsRNA-ssRNA junction-specific molecular glues for the probing and targeting of many biologically and medically important RNA structures in transcriptomes. I will discuss the applications of the PNAs platform in targeting a miRNA hairpin precursor, a tau pre-mRNA splice site hairpin structure, and an influenza viral RNA structure.

 

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